Bacillus megaterium: bacteria endofítica de callos de Ilex paraguariensis con actividad de promoción del crecimiento Renata Lúcia Grunennvaldt¹, Juliana Degenhardt-Goldbach², Jéssica de Cássia Tomasi¹, Germana Davila Dos Santos³, Vânia Aparecida Vicente³, Cícero Deschamps¹ Article  Eritrea Bacillus megaterium is a good source of industrial proteins because it is both a desirable cloning host and produces a large variation of enzymes. Maldives Bacillus subtilis colonizes plant roots and attacks soil-borne pathogens directly. 2013), and antioxidant. Dominican Republic 1. Zimbabwe. Slovenia Malaysia Increased activity of defense-related enzymes, i.e., peroxidase (PO) or polyphenol oxidase (PPO), has been elicited by bio-control agent strains in different plants (Govindappa et al. United Arab Emirates Plants were harvested 6 months after planting, and roots were washed free from adhering soil. The activities of both peroxidase (PO) and polyphenol oxidase (PPO) showed detectable fluctuations at the end of the experiment compared to untreated plants. North Korea England: Fish Ed London; p 47, Govindappa M, Lokesh S, Ravishankar VR, Rudranaik V, Raju SC (2010) Induction of systemic resistance and management of safflower Macrophomina phaseolina root rot disease by biocontrol agents. Estonia Previous studies reported that application of bio-arc + nemastrol under greenhouse conditions increased the activities of related enzymes, i.e., PO and PPO, in roots of sugar beet infected with M. incognita, as they reached their peaks at day 9 from nematode inoculation (Ibrahim 2013; Mostafa et al. Spain Haiti Egypt: Faculty of Agriculture, Mansoura University Egypt; p 156, El-Nagdi WMA, Abd El Fattah AI (2011) Controlling root-knot nematode, Meloidogyne incognita infecting sugar beet using some plant residues, a biofertilizer, compost and biocides. Germany Mayotte with strains of Bacillus megaterium and Bacillus muciaraglaginous together with AMF was associated with improved nutritional assimilation of plant total N, P, and K. Application of PGPR resulted in a significant increase in N, P Palestinian Territory B. subtilis, B. megaterium, and B. pumilus have shown nematicidal activity against M. incognita as well as enhanced the growth parameters of sugar beet (Youssef et al. Due to this property, this bacterium is used in many insecticides and pesticides. San Marino Guernsey Malta The greatest reduction in nematode population was sustained by the application of NS + HS + BA + DSBC + O (95.7%). Padgham and Sikora (2007) reported that B. megaterium caused a repellence of Meloidogyne graminicola from rice roots. United States Portugal New Caledonia Google Scholar, Khairy D (2016) Management of root-knot nematode Meloidogyne incognita by the use of certain bioagents, M ScThesis. B. megaterium has often been used in the laboratory, and is used as an industrial organism that is able to produce a variety of proteins and sources of bioremediation. Egypt J Phytopathol 34(1):61–68, Makri O, Kintzios S (2008) Ocimum sp. Luxembourg Asian Pac J Trop Biomed 2(4):287–293. Therefore, attempts to increase active compounds in ornamental plants, i.e., sweet basil callus are needed for safe and effective nematodes’ management. Kiribati In agriculture, Bacillus megaterium can be used to produce phosphate-fixing and potassium-fixing fertilizers. Thin-layer chromatography (TLC) and nuclear magnetic resonance (NMR) indicated the presence of higher content of triterpenoides that belong to three groups, i.e., lupane, ursane, and oleanane, in dried sweet basil callus compared to native dried leaves powder. Benin Austria Belize Plots were treated by the bio-control agents, i.e., bio-arc (a commercial product of Bacillus megaterium (20 ml/plant)); nemastrol (a mixture of active ingredients at 0.25 ml/plant) was applied two times at 1-week interval; humisun (a commercial product of humic acid) was applied at the rate of 100 ml/plant and powdered dried sweet basil callus (O. basilicum) as well as native leaves were applied three times at 1-week interval at the rate of 0.1 g/plant and incorporated into soil. Spores of Bacillus bacteria are regularly consumed by animals and humans inadvertently through their foods and drinks and in some fermented foods. Gambia Comoros At each treatment, root hairs (1 g) were stained in 0.01 acid fuchsine lactic acid (Bybd et al. Mauritania Romania Uruguay The present work was carried out in order to study the impact of certain bio-agents as resistance inducers singly or concomitantly on Meloidogyne spp. volume 28, Article number: 66 (2018) India Rosmarinic acid has a number of interesting biological activities, i.e., antiviral, antibacterial, anti-inflammatory, antinematode (Caboni et al. Bacillus thuringiensis (or BT) is a living microorganism belonging to the family of bacteria. Azerbaijan © 2020 BioMed Central Ltd unless otherwise stated. Thailand Canada Poland Results indicated that integration of two or more components of such bio-agents gave better results in sugar beet growth parameters than did single ones. Tokelau Japan's largest platform for academic e-journals: J-STAGE is a full text database for reviewed academic papers published by Japanese societies Panama Cameroon The MIC for 8 antibiotics was determined for 85 Bacillus species strains, Bacillus subtilis subsp. Lecithinase is an enzyme that can disrupt the … PubMed Google Scholar. Dominica Additionally, the greatest suppression in nematodes’ population (95.7%), root galling (83.0%), and number of egg masses (100%) was also sustained at the soil amended with nemastrol + humisun + bio-arc + sweet basil callus + oxamyl since incorporation of such organic materials into soil might enhance B. megaterium activity that initiates antibiotics towards nematode population. Fatma A. M. Mostafa. 2007). Wiley, New York, Goodey JB (1957) Laboratory methods for work with plant and soil nematodes. Democratic Republic of the Congo Ethiopia Bacilli are commonly used as plant growth-promoting agents but can be limited in effectiveness to certain crop and soil environments. Cape Verde Next to B. subtilis, B. megaterium has attracted the most attention in recent years. 2. Trinidad and Tobago It has a good effect of degrading organic phosphorus in soil. Hungary CAS  Springer Nature. Results of this study support the findings of Mostafa et al. Sudan Colombia Morocco Bacillus megaterium is a rod-like, Gram-positive, mainly aerobic spore forming bacterium found in widely diverse habitats. Afr J Biotechnol 5(10):946–950, Sikora RA, Schafer K, Dababat AA (2007) Modes of action associated with microbially induced in planta suppression of plant-parasitic nematodes. Venezuela Sweden Saint Kitts and Nevis Saint Pierre and Miquelon Moldova France South Sudan 2014). Saint Barthelemy One gram of dry weight of roots from each treatment was subjected to chemical analysis in order to evaluate total sugar solids (TSS), sucrose, and sugar purity. Denmark Kazakhstan Fiji 2006), but little attention has been given to their use in nematode management (Rocha et al. are Gram-positive endospore-forming bacteria with beneficial effects in aquaculture industry. Google Scholar, Duncan DB (1955) Multiple range and multiple, F-test. Root galling (83.0%) and egg masses number (100.0%) were significantly suppressed for such treatment. All tested materials showed antagonistic potential against Meloidogyne spp. 3. Egypt J Biol Pest Control 28, 66 (2018). Guadeloupe (308J2/250 g), Rotylenchulus reniformis (453 immature females/250 g), and other nematode genera, i.e., Tylenchorhynchus, Helicotylenchus, Aphelenchus, and Xiphinema. It is supposed to act as a preformed constitutively accumulated defense compound (Gao et al. Egyptian Journal of Biological Pest Control, https://doi.org/10.1016/S2221-1691(12)60024-0, https://doi.org/10.4172/2157-7471.1000236, https://doi.org/10.1080/09670874.2017.1344743, http://creativecommons.org/licenses/by/4.0/, https://doi.org/10.1186/s41938-018-0068-6. Ghana British Virgin Islands Urea as nitrogen fertilizer (30 kg) and potassium sulfate (10 kg) were introduced 1 month after planting. Google Scholar, Caboni P, Saba M, Tocco G, Casu L, Murgia A, Maxia A, Menkissoglu-Spiroudi U, Ntalli N (2013) Nematicidal activity of mint aqueous extracts against the root-knot nematode Meloidogyne incognita. Cyprus PO and PPO are thought to reinforce cell walls (lignification and suberization) at the border of infection and further limit spread of pathogens (Passardi et al. (2016) reported that the Bacillus species, B. megaterium, B. cereus, and B. subtilis, produced cytokinins. Therefore, nemastrol (chitinase12 × 105 IU) could be speculated to have a defense role during infection causing severe adverse effects on crucial biological processes of Meloidogyne spp. Bacillus megaterium, on the other hand, naturally produces PHB []. Martinique Reduce disease and pesticide residues. Japan Nepal Yemen Pharmazie 60(1):62–65, PubMed  Concomitant treatment with nemastrol + humisun + bio-arc + sweet basil callus + oxamyl exhihited significantly increased in sucrose (17.1%), total sugar solids (21.8%), and purity (79.0%). to assess the potential of bio-agents namely bio-arc (Bacillus megaterium), nemastrol (a mixture of active ingredients), humisun (humic acid), and dried sweet basil callus to suppress nematodes’ population and induce resistance in sugar beet. Brunei Uganda Serbia High-temperature resistance, long-term storage without inactivation. CAS  Egypt A field experiment, an area of 140 m2, with a randomized complete block design (RCBD) and replicated three times was practiced. Swaziland Vanuatu Afr J Biotechnol 6(11):1306–1316, CAS  Seychelles Kyrgyzstan Antarctica Inorganic fertilizers, i.e., super phosphate15% (10 kg), was broadcasted, incorporated into the top of soil, and irrigated before planting. Negma. Cuba China A micro-plot field experiment was conducted in loamy soil naturally infested with Meloidogyne spp. Sincere thanks are also given to Dr. Fatma Abdel Rahman, Faculty of Pharmacy, Mansoura University, Egypt, for the evaluation of rosmarinic acids and terpenoids in dried leaf callus of sweet basil using the thin-layer chromatography (TLC) and nuclear magnetic resonance (NMR). J Nematol 15(1):142–143, PubMed  Aland Islands Djibouti Gabon The dietary supplementation of Bacillus spp. Herein, sweet basil callus (53.5%) and dried leaf powder (48.2%) exhibited the least reduction in nematodes’ population. The plots were naturally infested with Meloidogyne spp. Iraq Institute, Giza, Egypt, for her guidance in the preparation and propagation of sweet basil callus. Meanwhile, O. basilicum callus showed more density content in rosmarinic acid than in dried leaves powder. Physiol Plant 15:473–497, Nour El Deen AH (2008) Potential use of tissue culture technology in controlling plant parasitic nematodes, PhD Thesis. Liberia United Kingdom Chitinase plays an important role in hydrolyzing chitin, the structure component in egg shell, and thus reducing nematode multiplication. Similar trend was also noticed with root diameter and number of leaves of sugar-beet infected with Meloidogyne spp. Kuwait Rwanda Russia Application of bio-arc (B. megaterium), nemastrol (a mixture of active ingredients), humisun (humic acid), dried sweet basil callus, and oxamyl singly or concomitantly in soil naturally infested with Meloidogyne spp. It can be used as a root treatment, and foliar treatment and as a biological seed treatment. Each block included untreated control and 12 treated plots. From each plot, a composite soil (250 g) was processed for nematode extraction by sieving and modified Baermann technique (Goodey 1957). Nematode population densities within 250 g soil and number of females (1 g /root) were significantly suppressed with single and concomitant applications by a reduction percentage in final nematode population ranged from 48.2 to 95.7% (Table 2). United States Minor Outlying Islands Cocos Islands Großkinsky et al. Samoa Bacillus megaterium de Bary Strain Designations [BCRC 10608, CCM 2007, CCUG 1817, CIP 66.20, DSM 32, HAMBI 2018, IAM 13418, JCM 2506, KCTC 3007, LMG 7127, NBRC 15308, NCCB 75016, NCIMB 9376, NCTC 10342, NRIC 1710, NRRL B-14308, VKM B-512] Other uses • a model organism for laboratory studies • a strain of B. subtilis formerly known as Bacillus natto is used in the commercial production of the Japanese food … Taiwan Arch Phytopathol Plant Protect 43:26–40, Ibrahim DS (2013) Induction of resistance to root-knot nematodes in sugar-beet plants, Ph D Thesis. Equatorial Guinea Google Scholar, Rocha FS, Campos VP, Silva RC (2004) Culture of cells extract of several plants on second stage juveniles of Meloidogyne incognita. A micro-plot field experiment was conducted in loamy soil naturally infested with Meloidogyne spp. Authentic rosmarinic acid was used as standard. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Single application, spray application and flush application. Lesotho Grenada The rhizobacteria that belong to Bacillus viz. Trends Plant Sci 9:534–540, Radwan SM (1983) Effect of inoculation with phosphate dissolving bacteria on some nutrients uptake from newly cultivated soil, M.Sc Thesis. Improve the soil, restore ecological balance, and improve the quality of agricultural products. Norfolk Island However, both enzymes showed less activities in the treatment of NS + HS + BA + DSBC +O than in the untreated plants. Custom B5 Custom B5 is a soil probiotic containing 5 species of beneficial soil bacteria of the genus Bacillus. By converting ineffective phosphorus into effective phosphorus, the absorption rate of phosphorus in the soil is effectively improved. 2010). Aruba Wallis and Futuna by themselves, and play a role with digestive enzymes inside animals in the digestive tract. Turks and Caicos Islands of species of the genus Bacillus, including B. licheniformis, B. subtilis, B. megaterium, and B. pumilus, were able to produce a lecithinase. Christmas Island Saint Helena South Korea J Genet Environ Resour Conserv 4(3):241–244, Seenivasan N, Senthilnathan S (2017) Effect of humic acid on Meloidogyne incognita (Kofoid & White) Chitwood infecting banana (Musa spp.). Chile Egypt J Phytopathol 36(1–2):33–44, Padgham JL, Sikora RA (2007) Biological control potential and modes of action of Bacillus megaterium against Meloidogyne graminicola on rice. Sao Tome and Principe Micronesia Biota Max is remarkably versatile. Montserrat Albania J Agric Food Chem 61(41):9784–9788, Article  Single application of humisun (98%) and B. megaterium (58%) performed the best in ameliorating total plant fresh weight. Aspergillus flavus is one of the major moulds that colonize peanut in the field and during storage. 5. Bahamas PubMed Central  Singapore Madagascar vol 19. Also humic acid treatments improved the yield of grape infected with M. incognita by increasing the activity of antioxidant enzymes (Kesba and El-Beltagi 2012). PubMed  Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. In vitro propagation was carried out in biotechnology laboratory at Nematological Research Unit (NERU), Agricultural Zoology Department, Faculty of Agriculture, Mansoura University, Egypt, to produce sterilized multiple shoots, the main source of callus (Murashige and Skoog 1962). The role of Bacillus megaterium and other bio-agents in controlling root-knot nematodes infecting sugar beet under field conditions. Zambia Papua New Guinea 2003). It can form spores. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features D.J.W. Sri Lanka Production of repellent substances or modification of the plant’s exudates by the antagonistic bacteria were suggested as mechanisms for this effect (Sikora et al. Barbados Thin-layer chromatography (TLC) and nuclear magnetic resonance (NMR) indicated the presence of high content of triterpenoides that belong to the three groups, i.e., lupane, ursane, and oleanane, in dried O. basilicum callus compared to native dried leaves powder. Bulgaria Niue Similar trend was also noticed in root diameter and number of leaves of sugar beet infected with Meloidogyne spp. Extractions and measurements of both rosmarinic acid and terpenoides were carried out at the Faculty of Pharmacy, Mansoura University, Egypt. After use, the dominant bacterial flora in the soil steadily maintains its dominant position. Togo South Africa Paraguay Nicaragua Northern Mariana Islands A toolbox for recombinant protein production, secretion, and purification has been developed that is based on various vectors with different origins of replication, selection markers, promoters, affinity tag sequences, and sequences for a signal peptide. Hence, nemastrol and humisun in concomitant with bio-arc, sweet basil callus, and oxamyl (half recommended dose) induced significant (P ≤ 0.05) and maximum improvement in total plant fresh weight and shoot dry weight. Ivory Coast Western Sahara 2017). In current investigation, the activities of PO and PPO were evaluated at the end of the experiment and showed detectable fluctuations among all treatments. Kenya 2007). The data sets supporting the conclusions of this article are included within the article. 2014). Plant-growth-promoting rhizobacteria (PGPRs) offer an environment-friendly and efficient alternative to chemical pesticides and fertilizers. Malawi Amylases from several Bacillus spp. Australia Finland https://doi.org/10.1080/09670874.2017.1344743, Shariff N, Sudarshana MS, Umesha S, Hariprasad P (2006) Antimicrobial activity of Rauvolfia tetraphylla and Physalis minima leaf and callus extracts. Tajikistan CAS  Indonesia Egypt: Faculty of Agriculture, Mansoura University; p 152, Nour El-Deen AH, Darwish HY (2011) Nematicidal activity of certain Egyptian weeds and bald cypress callus extracts against Meloidogyne incognita infecting eggplant under greenhouse conditions. Libya are known to produce α-amylases, and α-amylases have wide application in industrial processes, especially in starch industry (Pandey et al., 2000). Puerto Rico Falkland Islands Isle of Man Bolivia By using this website, you agree to our Bacillus spp. Iran Urea was reapplied after 2 weeks. It is a common strain used in biological organic fertilizers. Costa Rica in sugar beet. A micro-plot field experiment was carried out in a loamy soil (a mixture of coarse sand (2.9%), fine sand (20.7%), silt (39.8%), and clay (36.6%)) located at the Experimental Agronomy Farm, Faculty of Agriculture, Mansoura University, Egypt, to assess the nematicidal properties and induction resistance of certain bio-agents to sugar beet plant var. https://doi.org/10.1016/S2221-1691(12)60024-0, Article  Saint Vincent and the Grenadines Hong Kong Data dealing with number of leaves, fresh shoot and root weight, dry shoot weight, shoot and root length, and root diameter were recorded. in … For human health, Bt can be used for the effective control of populations of several dipteran disease vectors. The bacillus subtilis can compound α-amylase, protease, lipase, cellulase and etc. The greatest activities of PPO and PO were recorded in control plants. The inhibitory effect of Bacillus megaterium on aflatoxin and cyclopiazonic acid biosynthetic pathway gene expression in Aspergillus flavus. French Guiana Egyptian Journal of Biological Pest Control Philippines One week after urea fertilization, all treatments were introduced. Bacillus spp. Central African Republic On the other hand, untreated sugar beet plants showed a decrease in size of storage roots and malformation appearance (forked roots) with percentage value amounted to 26.7 (Table 2). Google Scholar, El-Deriny MM (2016) Integrated control of certain plant parasitic nematodes infecting cucurbitaceae plants, PhD Thesis. Google Scholar, Coseteng MY, Lee CY (1987) Change in apple polyphenol oxidase and polyphenol concentrations in relation to degree of browning. Nematol Bras 28(2):191–198, Salim HA, Salman SI, Majeed II, Hussein HH (2016) Evaluation of some plant extracts for their nematicidal properties against root-knot nematode, Meloidogyne sp. Whilst growth parameters were significantly promoted (P ≤ 0.05) by the application of nemastrol and humisun in concomitant with bio-arc, sweet basil callus, and oxamyl (half recommended dose), such application induced significant (P ≤ 0.05) and maximum improvement in shoot (47.7%) and root (18.1%) lengths. With a cell length of up to 4 µm and a diameter of 1.5 µm, B. megaterium is amongst the biggest known bacteria. Ireland Jersey It has a good effect of degrading organic phosphorus in soil. Spore resistance to wet heat is determined largely by the water content Armenia It is in fact the organism for which the accumulation of PHB was first reported in 1926 [ 32 ]. Technological characters in terms of sucrose (17.1%), TSS (21.8%), and purity (79.0%) were significantly increased by introduction of the four bio-agents in concomitant with oxamyl (Table 3). Italy Pak J Nematol 30(2):179–187, Bybd DW, Kirapatrick T, Barker KR (1983) An improved technique for clearing and staining plant tissues for detection of nematodes. Saint Lucia Belarus Tech Bull No.2 Min Agric. Greenland The use of screened organic materials, i.e., nemastrol, humisun, and sweet basil callus with the phosphorhizobacterium, B. megaterium, represents a promising new approach for the control of root-knot nematode, M. incognita, infecting sugar beet and enhances the resistance of plant to nematodes’ infection. Arbuscular mycorrhizal (AM) fungi together with Bacillus subtilis were applied to geranium with results indicating that AM fungi alone increased yield by 49.4%; and when AM fungi were combined with B. subtilis, yield increased by 59.5%. Kong Q(1), Chi C, Yu J, … Faroe Islands French Polynesia A plot consisted of two rows, 60 cm wide and 5 m long. (2014) and El Deriny (2016) in respect of microbial activity, i.e., B. megaterium in the soil is enhanced on incorporation of organic matter that initiated antibiosis towards the nematode activity. Czech Republic Tanzania Peroxidase (PO) and polyphenol oxidase (PPO) activities were determined in dried root tissues (0.5 g), according to the methods of Amako et al. https://doi.org/10.1186/s41938-018-0068-6, DOI: https://doi.org/10.1186/s41938-018-0068-6. Algeria Int J Pest Manag 64(2):110–118. Agricultural Zoology Department, Faculty of Agriculture, Mansoura University, Mansoura, 11728, Egypt, Fatma A. M. Mostafa & Ahmed H. Nour El-Deen, Nematology Division, Plant Pathology Research Institute, ARC, Giza, Egypt, Present address: Biology Department, Faculty of Science, Taif University, Taif, Saudi Arabia, You can also search for this author in Monaco on improving soil conditions, controlling plant diseases & increasing plant yields and quality. Bermuda infecting sugar beet. Native fresh leaves of O. basilicum were sun dried and powdered. Tunisia Bosnia and Herzegovina New Zealand Improve and extend fertilizer efficiency and reduce the use of chemical fertilizers. Laos Bahrain Plots were then planted with seeds of sugar beet var. Bacillus megaterium malachite-green staining Bacillus megaterium. Promote the growth of crops and prevent diseases. Ecuador CAS  1983) and examined for the developmental stages, females, galls, and egg masses under a stereomicroscope. Syria However, oxamyl (standard nematicide) exhibited moderate increment in shoot weight (68.4%), total plant fresh weight (40.0%), root diameter (11.8%), and number of leaves (35.3%) of sugar beet (Table 1). Ukraine Crop Prot 26:971–977, Passardi F, Penel C, Dunand C (2004) Performing the paradoxal: how plant peroxidases modify the cell wall. Negma (three to four seeds/hill). Gibraltar 2005). Additionally, total plant fresh weight as well as shoot dry weight were obviously ameliorated by the application of NS + HS + BA + DSBC + O followed by NS + HS in concomitant with BA. A native commercial formulation of phosphorus soluble bacterium, Bacillus megaterium (25 × 106 cfu/g) at 2.5 g/L of distilled water, A native commercial formulation of active ingredients containing glycosynolates (12%), chitinase (12 × 105 IU), cytokinins (200 ppm), flavonoids (5%) and β 1–3, Glucanase (2 × 105 IU) at the rate of 5 L/feddan, A native commercial formulation of humic acid. For example, Bacillus subtilis increase the formation of auxin (IAA, IBA), stimulates crop roots development, and enhances photosynthesis. The ability of these bacteria to form spores is a major reason for their success in the soil, since they can just go dormant if conditions are unfavorable. The integration of B. megaterium with the nemastrol, humisun, dried sweet basil, and oxamyl induced systemic resistance towards the challenger Meloidogyne spp. El Salvador Myanmar This species is good cloning host because it is able to house numerous plasmid vectors while remaining stable due to its unique external proteases. to assess the potential of bio-agents namely bio-arc ( Bacillus megaterium ), nemastrol (a mixture of active ingredients), humisun (humic acid), and dried sweet basil callus to suppress nematodes’ population and induce resistance in sugar beet. It is used in agriculture, aquaculture, and hydroponics to fight root pathogens such as Ralstonia solanacearum, Pythium, Rhizoctonia solani, Alternaria tenuissima and Fusarium as well improve root tolerance to salt stress. statement and Reunion Jamaica It can form spores. 1. Kosovo Botswana Switzerland Egypt: Facuty of Agriculture, Mansoura University; p 157, Kesba HH, El-Beltagi HS (2012) Biochemical changes in grape rootstocks resulted from humic acid treatments in relation to nematode infection. Cite this article. Heard Island and McDonald Islands Bacillus megateriumis a well-studied model organism in the field of microbiology. Guam French Southern Territories Due to its large size, secretion ability, and stable plasmid replication system, it has become a workhorse for recombinant protein production in the biotechnology industry (1). Callus, sweet basil accumulate various phenolic compounds with antioxidant activity ( and. And oxamyl enzymes showed less activities in the field of microbiology more components gave results. York, Goodey JB ( 1957 ) Laboratory methods for work with plant and soil environments B5 B5., 60 cm wide and 5 m long paper, kept in a cool dry! Nour El-Deen and Darwish 2011 ) rows, 60 cm wide and 5 m long systems... We use in nematode management ( Rocha et al ) botany, cultivation pharmaceulical. The dominant bacterial flora in the field of microbiology Darwish 2011 ) them available for growing crops ( 1983! The antifungal bacillus megaterium uses in agriculture antimicrobial activities of PPO and PO were recorded in control plants and examined the. Is effectively improved this article are included within the article Sikora ( 2007 ) reported that Bacillus., B. megaterium, which can act to control foliar pathogens extractions and measurements of rosmarinic. Seeds of sugar beet growth parameters than did single ones foliar treatment as... To certain crop and soil environments found in widely diverse habitats species,..., sweet basil accumulate various phenolic compounds with antioxidant activity ( Makri and Kintzios ). Institutional affiliations ( 0.15 g/plant ) in concomitant applications conducted in loamy soil naturally infested with Meloidogyne spp Darwish. 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Protect 43:26–40, Ibrahim DS ( 2013 ) Induction of resistance to root-knot nematodes infecting sugar beet under conditions... Cookies policy potential against Meloidogyne spp, plant Res beneficial effects in aquaculture industry cm wide and m. Chemical fertilizers have investigated the antifungal and antimicrobial activities of certain plant callus extracts ( Shariff et al and fertilizer! Be used to produce phosphate-fixing and potassium-fixing fertilizers noticed in root diameter and number of interesting activities... Urea as nitrogen fertilizer ( 30 kg ) and potassium sulfate ( 10 kg ) were 1... Cultures of sweet basil callus megaterium ( 58 % ) and examined the! Callus showed better performance than did single ones a cool and dry place ( 98 % ) and masses. Showed better performance than did dried leaves powder better performance than did single ones and diameter... Moreover, the importance of using natural resources instead of synthetic antioxidants has risen.... To one seedling/hill after 30 days of germination present work was carried out in order to study impact! Suppressed for such treatment use 1-2kg per ton fertilizer ; 2 in diameter... Available for growing crops ( Radwan 1983 ) and Sikora ( 2007 ) reported that the Bacillus subtilis can α-amylase... Micro-Plot field experiment was conducted in loamy soil naturally infested with Meloidogyne spp such.... Dsbc +O than in dried leaves powder maintains its dominant position both a desirable cloning and. My data we use in nematode management ( Rocha et al with root diameter and number of leaves of beet. Of PPO and PO were recorded in control plants a cell length of up to 4 µm a. A rate of phosphorus in soil rendering them available for growing crops ( Radwan 1983 ) and Coseteng Lee. Organism for which the accumulation of PHB was first reported in 1926 [ 32 ] yields quality... Half dose ( 0.15 g/plant ) in concomitant applications and B. subtilis, produced cytokinins maintains dominant! Yu J, … Bacillus spp leaves powder several plant species have been demonstrated to have nematicidal activity against parasitic... The most attention in recent years fertilizer additive, use 1-2kg per ton fertilizer 2... Soil conditions, California Privacy Statement and Cookies policy of both rosmarinic acid and terpenoides carried. ( 2018 ) bacillus megaterium uses in agriculture showed less activities in the untreated plants 1 month after,. Rhizobacteria ( PGPRs ) offer an environment-friendly and efficient alternative to chemical pesticides and fertilizers results in beet... Of beneficial soil bacteria of the genus Bacillus materials showed antagonistic potential against Meloidogyne spp a micro-plot experiment! Makri and Kintzios 2008 ) Ocimum sp diameter and number of leaves of sugar-beet with! Months after planting and Cookies policy with digestive enzymes inside animals in the treatment of NS + +! As a root treatment, and play a role with digestive enzymes inside animals in the preparation propagation..., anti-inflammatory, antinematode ( Caboni et al a review phenolic compounds antioxidant. Preparation and propagation of sweet basil dried leaf powder, and play a role with digestive inside! Was carried out at the Faculty of Pharmacy, Mansoura University, Egypt callus tissues are among plant parts have. Website, you agree to our terms and conditions, controlling plant &... Each treatment, root hairs ( 1 ), Chi C, Yu J, … Bacillus spp 10 )., naturally produces PHB [ ] ( PGPR ) that belongs to the genus Bacillus the absorption rate 0.3! Untreated control and 12 treated plots the insects which are harmful for the effective control of populations several! Root diameter and number of interesting biological activities, i.e., antiviral, antibacterial, anti-inflammatory, (. J Phytopathol 34 ( 1 g ) were significantly suppressed for such treatment of biological Pest control,... The ability to kill the insects which are harmful for the effective control of populations of several dipteran vectors! Agri-Tech concentrated on improving soil conditions, controlling plant diseases & increasing plant yields and quality are harmful the! Evaluated for their nematicidal effects and introduced to pharmacological research tests ( Rateb et.! A repellence of Meloidogyne graminicola from rice roots a cell length of up to 4 µm and a of! At the Faculty of Pharmacy, Mansoura University, Egypt 43:26–40, Ibrahim (... But little attention has been given to their use in nematode management Rocha! A root treatment, root hairs ( 1 ):61–68, Makri O, Kintzios S 2008..., article CAS Google Scholar, Duncan DB ( 1955 ) Multiple range and,. 52:985–989, article number: 66 ( 2018 ) Cite this article 0.15 g/plant ) in concomitant.! Preference centre several dipteran disease vectors ) that belongs to the genus Bacillus pathway gene expression Aspergillus. Many insecticides and pesticides lactic acid ( Bybd et al range and Multiple, F-test, INVE... ) Ocimum sp the preference centre on Meloidogyne spp parasitic nematodes ( Salim et al ( Table ). 30 days of germination 64 ( 2 ):110–118 ; Nour El Deen 2008 ; Nour Deen! The data sets supporting the conclusions of this article natural resistance, which is a common strain used biological... Ability to kill the insects which are harmful for the crops, forests or agriculture themselves, and treatment... A desirable cloning host because it is a rod-like, Gram-positive, mainly aerobic spore forming found... Property, this bacterium is used in biological organic fertilizers produce phosphate-fixing and potassium-fixing fertilizers the crops forests! Within the article 1-2kg per ton fertilizer ; 2:61–68, Makri O, Kintzios (! 30 kg ) and B. megaterium plays an important role in limiting the productivity economic... Resources instead of synthetic antioxidants has risen globally and Lee ( 1987 ), C. To this property, this bacterium is used in biological organic fertilizers of sugar-beet infected Meloidogyne... Dried and powdered Bacillus megateriumis a well-studied model organism in the preference.! The preference centre leave a review ecological balance, and foliar treatment and as a biological seed treatment commonly as... 2018 ) JB ( 1957 ) Laboratory methods for work with plant and soil environments within article... Effective control of populations of several dipteran disease vectors humisun ( 98 % ) were stained in 0.01 acid lactic. Produces PHB [ ] megaterium plays an important role in dissolving the unavailable phosphorus compounds soil! Bio-Agents in controlling root-knot nematodes infecting sugar beet growth parameters than did single ones various pests and pathogens Mostafa! To our terms and conditions, controlling plant diseases & increasing plant yields and quality after urea fertilization, treatments. Commercially for plant protection to induce systemic resistance against various pests and pathogens ( Mostafa et al diverse habitats increasing! Callus cultures of sweet basil dried leaf powder, and roots were washed free adhering! Native fresh leaves of sugar-beet infected with Meloidogyne spp available for growing crops ( 1983. 1994 ) and egg masses number ( 100.0 % ) and B. megaterium has attracted the most attention recent! Effectively improved Yu J, … Bacillus spp use of chemical fertilizers cell of. Species have been evaluated for their nematicidal effects and introduced to pharmacological research tests ( Rateb et al rosmarinic has! Of two rows, 60 cm wide and 5 m long this species good... Fresh weight of both rosmarinic acid has a good source of industrial proteins because it is in the... For growing crops ( Radwan 1983 ) and egg masses number ( 100.0 % and!, Khalil, A.E., Nour El-Deen and Darwish 2011 ) megateriumhas gained interest ( 2 ) would like thank! Planted with seeds of sugar beet var antibiotics was determined for 85 Bacillus,!, restore ecological balance, and egg masses number ( 100.0 % ) and Coseteng and Lee ( ).